p3990 pET ubcH8
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8650||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3700
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneUBE2L6 (a.k.a. RIG-B, UBCH8)
/ Fusion Protein
- His (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3990 pET ubcH8 was a gift from Peter Howley (Addgene plasmid # 8650 ; http://n2t.net/addgene:8650 ; RRID:Addgene_8650)
For your References section:Physical interaction between specific E2 and Hect E3 enzymes determines functional cooperativity. Kumar S, Kao WH, Howley PM. J Biol Chem 1997 May 23;272(21):13548-54. 10.1074/jbc.272.21.13548 PubMed 9153201
Map uploaded by the depositor.