This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

p3521 pET ubcH6
(Plasmid #8653)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8653 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UbcH6
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBE2E1 (a.k.a. UBCH6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI? (unknown if destroyed)
  • 3′ cloning site XhoI? (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See author's map.

Cloning of this plasmid was described in the following pubication: https://www.ncbi.nlm.nih.gov/pubmed/8576257 J Biol Chem. 1996 Feb 2;271(5):2795-800.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3521 pET ubcH6 was a gift from Peter Howley (Addgene plasmid # 8653)
  • For your References section:

    Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. Scheffner M, Huibregtse JM, Howley PM. Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801. 10.1073/pnas.91.19.8797 PubMed 8090726