Addgene - p3521 pET ubcH6 Plasmid Data

Plasmid 8653: p3521 pET ubcH6

Gene/insert name:
UbcH6
Species:
H. sapiens (human)
Entrez Gene:
UBE2E1 (UBCH6)
Vector backbone:
pET-22a
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Bacterial Expression
Cloning site 5':
NdeI?
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI?
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Peter Howley
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

See author's map.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. Scheffner et al (Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8653" in your Materials and Methods section.