Addgene - p4054 HA-E6AP isoform II Plasmid Data

Plasmid 8658: p4054 HA-E6AP isoform II

Gene/insert name:
E6-AP isoform II
Alt name:
E6-AP
Species:
H. sapiens (human)
Entrez Gene:
UBE3A (ANCR, AS, E6-AP, EPVE6AP, HPVE6A)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pCMV4
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4874
Cloning site 5':
BamHI
Site destroyed during cloning:
Yes
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Peter Howley
Terms and Licenses:
MTA

Comments:

See author's map.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human papillomavirus type 16 E6 induces self-ubiquitination of the E6AP ubiquitin-protein ligase. Kao et al (J Virol 2000 Jul;74(14):6408-17. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8658" in your Materials and Methods section.