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pY036_ATP1A1_G5
(Plasmid #86618)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86618 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pY036-U6-crRNA(BbsI)-CBh-AsCpf1
  • Total vector size (bp) 8467
  • Vector type
    Mammalian Expression, CRISPR ; Co-selection via HDR using ouabain

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP1A1 G5 crRNA + AsCpf1-3xHA
  • Alt name
    hAsCpf1
  • Alt name
    Cpf1
  • Species
    Acidaminococcus_sp_BV3L6
  • Insert Size (bp)
    8467
  • Promoter CBh
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer agggatggttggttggtggg
  • 3′ sequencing primer CCAATCCTCCCCCTTGCTGT
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

crRNA target sequence is tagtacacatcagatatctt.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pY036_ATP1A1_G5 was a gift from Yannick Doyon (Addgene plasmid # 86618 ; http://n2t.net/addgene:86618 ; RRID:Addgene_86618)
  • For your References section:

    Marker-free coselection for CRISPR-driven genome editing in human cells. Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganiere J, Doyon Y. Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265. 10.1038/nmeth.4265 PubMed 28417998