pRN3P-HA-Suv4-20h2mut
(Plasmid #86692)

• Purpose: For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'
• Depositing Lab: Maria-Elena Torres-Padilla
• Publication: Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526. Epub 2016 Dec 5.

Backbone

• Vector backbone: pRN3p
• Backbone size w/o insert (bp): 3203
• Total vector size (bp): 4610

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: histone-lysine N-methyltransferase KMT5C mutant
• Alt name: Suv4-20h2 mutant
• Alt name: KMT5C
• Species: M. musculus (mouse)
• Insert Size (bp): 1421
• Mutation: Mutated asparagine 273 to cysteine 276 (NHDC) into AAAG (wild-type sequence CAACCATGACTG to mutated sequence CGCCGCTGCCGG)
• GenBank ID: AY555193.1
• Entrez Gene: Kmt5c (a.k.a. Suv4-20h2, Suv420h2)
• Promoter: T3
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xbai (not destroyed)
• 3′ cloning site: Noti (not destroyed)
• 5′ sequencing primer: T3 sequence
• 3′ sequencing primer: T7 sequence

Resource Information

• Supplemental Documents:
  • pRN3p-Suv4-20h2mut.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry