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pVENUS-N1-FNTA
(Plasmid #86859)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86859 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FNTA
  • Alt name
    Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
  • Alt name
    CAAX farnesyltransferase subunit alpha
  • Species
    H. sapiens (human)
  • Mutation
    The sequence of the insert is isoform 2, which differs from the canonical sequence as follows: 68-134: Missing.
  • GenBank ID
    NM_002027.2
  • Entrez Gene
    FNTA (a.k.a. FPTA, PGGT1A, PTAR2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-NR
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVENUS-N1-FNTA was a gift from Yihong Ye (Addgene plasmid # 86859 ; http://n2t.net/addgene:86859 ; RRID:Addgene_86859)
  • For your References section:

    The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation. Xu Y, Anderson DE, Ye Y. Cell Discov. 2016 Nov 8;2:16040. eCollection 2016. 10.1038/celldisc.2016.40 PubMed 27867533