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pcDNA5/FRT/TO-FNTB-HA
(Plasmid #86869)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86869 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone size w/o insert (bp) 5137
  • Total vector size (bp) 6448
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FNTB
  • Alt name
    Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit beta
  • Alt name
    CAAX farnesyltransferase subunit beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1311
  • Mutation
    none
  • GenBank ID
    NM_002028.3.
  • Entrez Gene
    FNTB (a.k.a. FPTB)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO-FNTB-HA was a gift from Yihong Ye (Addgene plasmid # 86869 ; http://n2t.net/addgene:86869 ; RRID:Addgene_86869)
  • For your References section:

    The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation. Xu Y, Anderson DE, Ye Y. Cell Discov. 2016 Nov 8;2:16040. eCollection 2016. 10.1038/celldisc.2016.40 PubMed 27867533