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pcDNA5/FRT/TO-GRB2-GFP
(Plasmid #86873)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86873 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA 5/FR/TO
  • Backbone size w/o insert (bp) 5070
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GRB2
  • Alt name
    SH2/SH3 adapter GRB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    651
  • Mutation
    none
  • GenBank ID
    NM_002086.4
  • Entrez Gene
    GRB2 (a.k.a. ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO-GRB2-GFP was a gift from Yihong Ye (Addgene plasmid # 86873 ; http://n2t.net/addgene:86873 ; RRID:Addgene_86873)
  • For your References section:

    The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation. Xu Y, Anderson DE, Ye Y. Cell Discov. 2016 Nov 8;2:16040. eCollection 2016. 10.1038/celldisc.2016.40 PubMed 27867533