Hygro-Cas9 donor
(Plasmid #86883)

• Purpose: Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
• Depositing Lab: Danwei Huangfu
• Publication: Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5909(17)30001-2. doi: 10.1016/j.stem.2017.01.001.

Backbone

• Vector backbone: N/A
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 Growth strain
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hSpCas9
• Species: Streptococcus pyogenes
• Insert Size (bp): 4101
• Tags / Fusion Proteins:
  • 3xFlag (N terminal on insert)
  • SV40 NLS (N terminal on insert)
  • Nucleoplasmin NLS (C terminal on insert)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry