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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepZLuc-TK
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3 STAT sites from the Ly6e promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)60
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJF Habener made the pZLuc vector (Biotechniques v7#10 (1989), p.1116)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
3 repeated STAT sites from the Ly6e promoter (Khan et al. PNAS 90:6806) cloned into TK-Luciferase (Shuai et al Science 261:1744). 2 STAT sites oriented 5'-TTN5AA-3' and 1 site oriented 5'-AAN5TT-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3xLy6e pZLuc-TK was a gift from Jim Darnell (Addgene plasmid # 8689) -
For your References section:
Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Wen Z, Zhong Z, Darnell JE Jr. Cell 1995 Jul 28;82(2):241-50. 10.1016/0092-8674(95)90311-9 PubMed 7543024
Map uploaded by the depositor.