hisSUMO-mZFP57
(Plasmid #87224)

• Purpose: Express hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coli
• Depositing Lab: Gary Stormo
• Publication: Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi: 10.1126/sciadv.aao1799. eCollection 2017 Nov.

Backbone

• Vector backbone: NEB DHFR control plasmid
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZFP57
• Species: M. musculus (mouse)
• Entrez Gene: Zfp57 (a.k.a. G19, Zfp-57)
• Promoter: T7
• Tag / Fusion Protein:
  • hisSUMO (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • hisSUMO-mZFP57.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct has the DNA binding domain of mouse ZFP57 including two separate groups of zinc fingers and the linker in between. In our published work about methylation sensitivity assay, only the truncated first group of Zinc finger (F1-F3) was used for Methyl-Spec-seq assay.