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pFG4
(Plasmid #87251)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87251 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAG32
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hyg
  • Alt name
    resistance to hygromycin
  • Promoter TEF
  • Tag / Fusion Protein
    • parS segments (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFG4 was a gift from Kerstin Bystricky (Addgene plasmid # 87251 ; http://n2t.net/addgene:87251 ; RRID:Addgene_87251)
  • For your References section:

    DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. Saad H, Gallardo F, Dalvai M, Tanguy-le-Gac N, Lane D, Bystricky K. PLoS Genet. 2014 Mar 13;10(3):e1004187. doi: 10.1371/journal.pgen.1004187. eCollection 2014 Mar. PGENETICS-D-13-01770 [pii] PubMed 24625580