pGL2-MCT2Prom3.2
(Plasmid #87349)

• Purpose: Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp
• Depositing Lab: Saroj Mathupala
• Publication: Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. doi: 10.1016/j.neo.2016.12.011.

Backbone

• Vector backbone: pGL2
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5598
• Total vector size (bp): 8765
• Vector type: Mammalian Expression, Bacterial Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable ; E. coli SURE for propagation, E. coli SCS110 for reporter studies
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCT2
• Alt name: SLC16A7
• Species: H. sapiens (human)
• Insert Size (bp): 3167
• Entrez Gene: SLC16A7 (a.k.a. MCT2)
• Promoter: 3.2 kbp proximal promoter of MCT2 (nested deletion construct)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (destroyed during cloning)
• 3′ cloning site: HinDIII (not destroyed)
• 5′ sequencing primer: GL primer 1
• 3′ sequencing primer: GL primer 2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A methylation deficient E. coli strain such as SCS110 is preferred to prepare plasmid DNA for mammalian cell transfection to reduce affects of prokaryotic methylation patterns on promoter activity.