PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||87351||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5598
- Total vector size (bp) 8030
Vector typeMammalian Expression, Bacterial Expression, Luciferase
Growth in Bacteria
Growth Strain(s)NEB Stable ; E. coli SURE for propagation, E. coli SCS110 for reporter studies
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2432
- Promoter 2.5 kbp proximal promoter of human MCT2 (nested deletion construct)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (destroyed during cloning)
- 3′ cloning site HinDIII (not destroyed)
- 5′ sequencing primer GL primer 1
- 3′ sequencing primer GL primer 2 (Common Sequencing Primers)
A methylation deficient E. coli strain such as SCS110 is preferred to prepare plasmid DNA for mammalian cell transfection to reduce affects of prokaryotic methylation patterns on promoter activity.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2-MCT2Prom2.5 was a gift from Saroj Mathupala (Addgene plasmid # 87351 ; http://n2t.net/addgene:87351 ; RRID:Addgene_87351)
For your References section:pH, Lactate, and Hypoxia: Reciprocity in Regulating High-Affinity Monocarboxylate Transporter Expression in Glioblastoma. Caruso JP, Koch BJ, Benson PD, Varughese E, Monterey MD, Lee AE, Dave AM, Kiousis S, Sloan AE, Mathupala SP. Neoplasia. 2017 Jan 13;19(2):121-134. doi: 10.1016/j.neo.2016.12.011. 10.1016/j.neo.2016.12.011 PubMed 28092823