pUC18-H1 RNAi
(Plasmid #87355)

• Purpose: RNAi expression vector based on H1 promoter and pUC18 vector backbone
• Depositing Lab: Saroj Mathupala
• Publication: Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; discussion 1419.

Backbone

• Vector backbone: pUC18
• Total vector size (bp): 2864
• Modifications to backbone: MCS is BglII-XbaI-HinDIII. H1 RNA promoter placed 5' to MCS.
• Vector type: Mammalian Expression, RNAi ; shRNA expression vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Can be maintained in E. coli SURE or any other recombination deficient strain
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H1 RNA Promoter from U87MG glioma cells
• Species: H. sapiens (human)
• Insert Size (bp): 219
• Promoter: H1 RNA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13 reverse sequencing primer
• 3′ sequencing primer: M13 forward sequencing primer

Resource Information

• Supplemental Documents:
  • sequence.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

H1 RNA promoter cloned from U87MG glioma (brain tumor) cells via RT-PCR. Vector suitable for shRNA studies, particularly for shRNA expression in glioma cells.