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2674 pBS Bcl-2 P1+P2
(Plasmid #8746)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8746 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II KS(-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl-2 P1+P2
  • Alt name
    Bcl-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5300
  • GenBank ID
    NM_000633
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

bcl-2 is from a genomic clone isolated from a normal human fibroblast library. To construct P1+P2Neo, a 279-bp polymerase chain reaction (PCR) product including the bcl-2 5'-untranslated region sequence from just 5' to the AccI site through base -1, relative to the initiation codon, (designated AccI/-1), was cloned into pBluescript KS(-) (Stratagene), using primergenerated 5' XhoI and 3' HindIII sites. Next, a 486-bp XhoI-AccI fragment from the 5'-untranslated region was cloned into this plasmid, producing a 756-bp XhoI/-1 (HindIII) clone. Addition of an approximately 2.2-kb XhoI fragment to this construct created a 3.0-kb bcl-2 P1+P2 clone. Finally, a 2.3-kb HindIII-BamHI fragment from RSVNeo (20) including the neomycin phosphotransferase (neo) gene and SV40 early splice and polyadenylation signals was inserted into the P1+P2 clone to produce P1+P2Neo.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2674 pBS Bcl-2 P1+P2 was a gift from Stanley Korsmeyer (Addgene plasmid # 8746 ; http://n2t.net/addgene:8746 ; RRID:Addgene_8746)
  • For your References section:

    A negative regulatory element in the bcl-2 5'-untranslated region inhibits expression from an upstream promoter. Young RL, Korsmeyer SJ. Mol Cell Biol 1993 Jun;13(6):3686-97. PubMed 8388542