3280 pcDNA3 Bid p22 cDNA
(Plasmid
#8767)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8767 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBid p22 ORF cDNA FL
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Alt nameBid
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SpeciesM. musculus (mouse)
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GenBank IDNM_007544
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Entrez GeneBid (a.k.a. 2700049M22Rik)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3280 pcDNA3 Bid p22 cDNA was a gift from Stanley Korsmeyer (Addgene plasmid # 8767 ; http://n2t.net/addgene:8767 ; RRID:Addgene_8767) -
For your References section:
BID: a novel BH3 domain-only death agonist. Wang K, Yin XM, Chao DT, Milliman CL, Korsmeyer SJ. Genes Dev 1996 Nov 15;10(22):2859-69. 10.1101/gad.10.22.2859 PubMed 8918887