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3336 pcDNA3 Bcl-2
(Plasmid #8768)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8768 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcl-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    720
  • GenBank ID
    NM_000633
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

ORF contains SacI, PstI, and BamHI sites sense from T7 direction.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3336 pcDNA3 Bcl-2 was a gift from Stanley Korsmeyer (Addgene plasmid # 8768 ; http://n2t.net/addgene:8768 ; RRID:Addgene_8768)
  • For your References section:

    BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M. Yamamoto K, Ichijo H, Korsmeyer SJ. Mol Cell Biol 1999 Dec;19(12):8469-78. PubMed 10567572