3540 pMIG Bid p15
(Plasmid #8789)

• Depositing Lab: Stanley Korsmeyer
• Publication: Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Backbone

• Vector backbone: pMIG
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bid p15
• Alt name: tBid
• Species: M. musculus (mouse)
• Insert Size (bp): 410
• GenBank ID: NM_007544
• Entrez Gene: Bid (a.k.a. 2700049M22Rik)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pLXSN 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

tBid can be cut out by EcoRI

Note that the GFP in this plasmid is not fused to gene. It is for selection purposes only.