3544 pMIG Bcl-2
(Plasmid #8793)

• Depositing Lab: Stanley Korsmeyer
• Publication: Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Backbone

• Vector backbone: pMIG
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Bcl-2
• Species: H. sapiens (human)
• Insert Size (bp): 720
• GenBank ID: NM_000633
• Entrez Gene: BCL2 (a.k.a. Bcl-2, PPP1R50)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the GFP in this plasmid is not fused to gene. It is for selection purposes only.

Please note that this plasmid is an extremely slow grower and may take up to 3 days to grow.