Addgene - pRK603 Plasmid Data

Plasmid 8831: pRK603

Gene/insert name:
TEV protease, S219D mutant
Alt name:
tobacco etch virus protease
Insert size:
700
Species:
tobacco etch virus
Mutation:
S219D mutant
Vector backbone:
pZA31
(Search Vector Database)
Backbone manufacturer:
Herman Bujard Lab
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
6700
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
pLTet-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
BL21-Pro
Growth temperature (℃):
37
Growth instructions:
For best results, perform intracellular processing experiments at 30C.
High or low copy:
Low Copy
Person or lab that originally
cloned the gene/insert:
ATCC
Sequence: View sequences (2)
Principal Investigator:
David Waugh
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

This TEV protease expression vector is intended to be used for controlled intracellular processing of fusion proteins (TEV protease substrates) in E. coli. Expression of the TEV protease gene on pRK603 will be constitutive unless the host strain produces Tet repressor. Regulated expression can be achieved in DH5alphaPro� or BL21Pro� cells (Clontech).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Controlled intracellular processing of fusion proteins by TEV protease. Kapust et al (Protein Expr Purif. 2000 Jul . 19(2):312-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8831" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

11086: pDEST-periHisMBP