pPS1622
(Plasmid #8854)

• Depositing Lab: Pamela Silver
• Publication: Prinz et al J Cell Biol. 2000 Aug 7. 150(3):461-74.

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 4895
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Sec63-sGFP
• Alt name: Sec63
• Alt name: pJK59
• Species: S. cerevisiae (budding yeast)
• Mutation: sGFP=S65T V163A mutant of GFP
• Entrez Gene: SEC63 (a.k.a. YOR254C, PTL1)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by amplifying SEC63 and ~400 bp of its 5' promoter from pPS599 (R. Schekman, UC Berkeley). Product cloned between XbaI and XhoI sites of CEN/URA3 plasmid, pJK50 (Kahana, MBC 1998).