3788 pmko-neo A9-1
(Plasmid #8878)

• Depositing Lab: Stanley Korsmeyer
• Publication: Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

Backbone

• Vector backbone: pmko-neo
• Backbone size w/o insert (bp): 7200
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hox A9
• Alt name: shRNA-HoxA9
• Species: H. sapiens (human)
• Entrez Gene: HOXA9 (a.k.a. ABD-B, HOX1, HOX1.7, HOX1G)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sequence in hairpin: CACGC TTGAC ACTCA CACTT T.