pSV Sport SREBP-1c
(Plasmid #8883)

• Depositing Lab: Bruce Spiegelman
• Publication: Tontonoz et al Mol Cell Biol. 1993 Aug . 13(8):4753-9.

Backbone

• Vector backbone: pSV-Sport
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3160
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SREBP-1c
• Alt name: ADD1
• Alt name: SREBP
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• Mutation: Active version of SREBP-1c.
• Entrez Gene: Srebf1 (a.k.a. ADD1, SREBP1, bHLHd1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that this is a mouse SREBP-1c, while the paper reference is a rat SREBP-1c. SREBP-1c differs from SREBP1a over amino acids 3-5 and contains a deletion of amino acids 6-29. See Figure 1c of the associated publication for more information.

Addgene's sequencing results identified a deletion of aa90-131 of SREBP1c compared to GenBank sequence NP_035610.1.