pcDNA3.1: mRmrp_G270T
(Plasmid
#88864)
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Purposeconstitutive expression of Rmrp G270T in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 88864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRmrp
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SpeciesM. musculus (mouse)
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Insert Size (bp)275
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MutationG270T
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GenBank IDNR_001460.1
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Entrez GeneRmrp (a.k.a. 1110032O22Rik, Rmrpr)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Pmel (not destroyed)
- 3′ cloning site Pmel (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHRev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1: mRmrp_G270T was a gift from Dan Littman (Addgene plasmid # 88864) -
For your References section:
DDX5 and its associated lncRNA Rmrp modulate TH17 cell effector functions. Huang W, Thomas B, Flynn RA, Gavzy SJ, Wu L, Kim SV, Hall JA, Miraldi ER, Ng CP, Rigo F, Meadows S, Montoya NR, Herrera NG, Domingos AI, Rastinejad F, Myers RM, Fuller-Pace FV, Bonneau R, Chang HY, Acuto O, Littman DR. Nature. 2015 Dec 24;528(7583):517-22. doi: 10.1038/nature16193. Epub 2015 Dec 16. 10.1038/nature16193 PubMed 26675721