p37-2iDMD-LR
(Plasmid #88892)

• Purpose: 37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
• Depositing Lab: Michele Calos
• Publication: Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/biot.201600477.

Backbone

• Vector backbone: p37
• Modifications to backbone: To lower copy number, reverted the svir002 high-copy mutation
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Plasmid is unstable during growth in bacteria. We recommend screening at least 3-5 small colonies immediately upon receipt by restriction digest and minimizing growth times.
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Dystrophin
• Alt name: DMD
• Species: H. sapiens (human)
• Insert Size (bp): 11262
• Entrez Gene: DMD (a.k.a. BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85)

Gene/Insert 2

• Gene/Insert name: luciferase
• Alt name: luc
• Species: Firefly
• Insert Size (bp): 1647

Gene/Insert 3

• Gene/Insert name: mCherry
• Alt name: red
• Insert Size (bp): 705

Resource Information

• Supplemental Documents:
  • p37-2iDMD-LR.gbk
• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: 10kb DMD core transferred from pDysE variant (Chapdelaine et al., Protein Eng 2000;13:611–5.)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry