pBABE puro PPAR delta
(Plasmid #8891)

• Depositing Lab: Bruce Spiegelman
• Publication: Brun et al Genes Dev. 1996 Apr 15. 10(8):974-84.

Backbone

• Vector backbone: pBABE puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPAR delta
• Alt name: PPARd
• Species: M. musculus (mouse)
• Insert Size (bp): 1320
• GenBank ID: U10375
• Entrez Gene: Ppard (a.k.a. NUC-1, NUC1, Nr1c2, PPAR-beta, PPAR-delta, PPAR[b], PPARdelta, Pparb, Pparb/d)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from pCMX-PPAR delta from Ron Evans
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry