Gal4 PGC1 alpha 1-550
(Plasmid #8894)

• Depositing Lab: Bruce Spiegelman
• Publication: Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Backbone

• Vector backbone: pCMX Gal4
• Backbone size w/o insert (bp): 4500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PGC-1 alpha
• Alt name: PPAR gamma coactivator 1
• Alt name: PGC1a
• Species: M. musculus (mouse)
• Insert Size (bp): 1650
• Mutation: amino acids 1-550 of PGC-1 alpha
• GenBank ID: NM_008904
• Entrez Gene: Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
• Tag / Fusion Protein:
  • Gal4 DBD (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: GAGTAGTAACAAAGGTCAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PGC1(1-550) was cloned downstream of the Gal4 DNA binding domain.