Addgene - pcDNA flag PPAR gamma Plasmid Data

Gene/insert name:		PPAR gamma
Alternative names:		PPARg
Insert size (bp):		1547
GenBank/Entrez ID of insert:		NM_011146
Gene/insert aliases:		Pparg, Nr1c3, PPARgamma, PPAR-gamma, PPAR-gamma2
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		flag
Terminal:		N terminal on insert
Vector backbone:		pcDNA3.1 myc-His A (Search Vector Database)
Backbone manufacturer:		Invitrogen
Type of vector:		Mammalian expression,Retroviral
Backbone size (bp):		5500
Cloning site 5':		NotI
Site destroyed during cloning:		No
Cloning site 3':		XbaI
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Principal Investigator:		Bruce Spiegelman
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAG_enhancer

315 - 602

CMV_immearly_promoter

236 - 852

CMV_fwd_primer

769 - 789

T7_promoter

863 - 881

Orf frame 2

983 - 2530

c_myc

2546 - 2575

6xHis

2591 - 2608

BGH_rev_primer

2648 - 2631

bGH_PA_terminator

2634 - 2861

f1_origin

2924 - 3230

pBABE_3_primer

3364 - 3344

SV40_enhancer

3565 - 3350

SV40_promoter

3362 - 3630

SV40_origin

3529 - 3606

SV40pro_F_primer

3591 - 3610

Orf frame 1

3745 - 4539

Neomycin

3748 - 4536

SV40_PA_terminator

4718 - 4837

EBV_rev_primer

4806 - 4825

M13_reverse_primer

4899 - 4881

M13_pUC_rev_primer

4920 - 4898

lac_promoter

4963 - 4934

pBR322_origin

5891 - 5272

Orf frame 1

6906 - 6046

Ampicillin

6906 - 6046

AmpR_promoter

6976 - 6948

Unique restriction sites

NdeI		484
KpnI		912
NotI		970
PmlI		2443
XbaI		2531
ApaI		2541
BstBI		2544
AgeI		2585
SmaI		3686
NarI		3873

Article: Degradation of the peroxisome proliferator-activated receptor gamma is linked to ligand-dependent activation. Hauser S et al. (J Biol Chem. 2000 Jun 16. 275(24):18527-33. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 8895" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.