tet operator
(Plasmid #8901)

• Depositing Lab: Lynda Chin
• Publication: Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

Backbone

• Vector backbone: pBluescript KS
• Backbone size w/o insert (bp): 3000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tet operator
• Species: E. Coli
• Insert Size (bp): 282

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

the tet operator can be released from this plasmid using a XhoI digest.

The construct is composed of the tet operator sequences followed by a CMV minimal promoter. The insert was digested with XhoI and EcoRI, klenow filled, and cloned into the SmaI site of pBluescript KS. See author's map for more information.