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Tyrosinase enhancer promoter
(Plasmid #8902)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8902 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone size w/o insert (bp) 12100

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tyrosinase enhancer & promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    9100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The entire insert containing both the tyr enhancer and promoter can be released with NotI and XhoI OR NotI and KpnI. See author's map for more information. Please also cite: Kaestner et al. Genes Dev 1997; 11(12):1583-95.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tyrosinase enhancer promoter was a gift from Lynda Chin (Addgene plasmid # 8902)
  • For your References section:

    Cooperative effects of INK4a and ras in melanoma susceptibility in vivo. Chin L, Pomerantz J, Polsky D, Jacobson M, Cohen C, Cordon-Cardo C, Horner JW, DePinho RA. Genes Dev. 1997 Nov 1. 11(21):2822-34. 10.1101/gad.11.21.2822 PubMed 9353252