Tyrosinase enhancer promoter
(Plasmid
#8902)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBluescript KS
- Backbone size w/o insert (bp) 12100
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametyrosinase enhancer & promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)9100
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The entire insert containing both the tyr enhancer and promoter can be released with NotI and XhoI OR NotI and KpnI. See author's map for more information. Please also cite: Kaestner et al. Genes Dev 1997; 11(12):1583-95.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tyrosinase enhancer promoter was a gift from Lynda Chin (Addgene plasmid # 8902) -
For your References section:
Cooperative effects of INK4a and ras in melanoma susceptibility in vivo. Chin L, Pomerantz J, Polsky D, Jacobson M, Cohen C, Cordon-Cardo C, Horner JW, DePinho RA. Genes Dev. 1997 Nov 1. 11(21):2822-34. 10.1101/gad.11.21.2822 PubMed 9353252
Map uploaded by the depositor.