pPS879
(Plasmid
#8906)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8906 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepPS811
- Backbone size w/o insert (bp) 6700
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNPL3 F160L
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)278
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MutationF160L
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Entrez GeneNPL3 (a.k.a. YDR432W, MTR13, MTS1, NAB1, NOP3)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GAL1 promoter, 2 micron plasmid. 278 bp BglII fragment from pPS877 in BglII linear pPS811.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPS879 was a gift from Pamela Silver (Addgene plasmid # 8906 ; http://n2t.net/addgene:8906 ; RRID:Addgene_8906) -
For your References section:
A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Lee MS, Henry M, Silver PA. Genes Dev 1996 May 15;10(10):1233-46. 10.1101/gad.10.10.1233 PubMed 8675010