FLAG-Skp2
(Plasmid #89142)

• Purpose: mammalian expression of human Skp2 with N-terminal FLAG tag
• Depositing Lab: Elizabeth Wilson
• Publication: Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9. doi: 10.1016/j.mce.2016.10.006. Epub 2016 Oct 6.

Backbone

• Vector backbone: pCMV5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Skp2
• Alt name: SKP2 isoform 1
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• GenBank ID: NM_005983.3
• Entrez Gene: SKP2 (a.k.a. FBL1, FBXL1, FLB1, p45)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results found an F331I mutation in Skp2 compared to NCBI reference sequence NP_005974.2. This mutation is not known to affect plasmid function.