pT7-VP6SA11
(Plasmid #89166)

• Purpose: Express Rotavirus SA11 VP6 in mammalian cells
• Depositing Lab: Takeshi Kobayashi
• Publication: Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 10.1073/pnas.1618424114.

Backbone

• Vector backbone: p3E5
• Backbone size w/o insert (bp): 3074
• Total vector size (bp): 4430
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rotavirus SA11 VP6
• Species: Rotavirus SA11
• Insert Size (bp): 1356
• GenBank ID: LC178568
• Promoter: T7 promotor

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 forward CTGTGGATAACCGTATTACCG
• 3′ sequencing primer: T7 terminal GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pT7_SA11_VP6.str
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry