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coel::GFP
(Plasmid #8937)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8937 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    unc-122 promoter
  • Alt name
    coel::GFP
  • Alt name
    unc-122
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    4000
  • Entrez Gene
    unc-122 (a.k.a. CELE_F11C3.2)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Marker for expression of GFP in coelomocytes. 4kb unc-122 promoter+ 4aa of 1st exon (8911-12903 of cosmid F11C3, start is at 12892) in Pst-BamHI sites of pPD95.77.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    coel::GFP was a gift from Piali Sengupta (Addgene plasmid # 8937 ; http://n2t.net/addgene:8937 ; RRID:Addgene_8937)
  • For your References section:

    Expression and function of members of a divergent nuclear receptor family in Caenorhabditis elegans. Miyabayashi T, Palfreyman MT, Sluder AE, Slack F, Sengupta P. Dev Biol. 1999 Nov 15. 215(2):314-31. 10.1006/dbio.1999.9470 PubMed 10545240