pCI flag PCAF
(Plasmid #8941)

• Depositing Lab: Yoshihiro Nakatani
• Publication: Yang et al Nature. 1996 Jul 25. 382(6589):319-24.

Backbone

• Vector backbone: pCI
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCAF
• Alt name: P/CAF
• Species: H. sapiens (human)
• Insert Size (bp): 2579
• GenBank ID: NM_003884
• Entrez Gene: KAT2B (a.k.a. CAF, P/CAF, PCAF)
• Tag / Fusion Protein:
  • flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to GenBank reference sequence NP_003875.3, Serine-2 is actually Valine-2 immediately after the FLAG tag.