p426-SNR52p-gRNA.clr-2.Y-SUP4t
(Plasmid #89692)

• Purpose: gRNA for clr-2 locus
• Depositing Lab: Christian Hong
• Publication: Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 10.1186/s40694-015-0015-1. eCollection 2015.

Backbone

• Vector backbone: pRS426
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 5726
• Total vector size (bp): 6274
• Modifications to backbone: The target sequence to csr-1 locus was added instead of CAN1 of p426-SNR52p-gRNA.CAN1.Y-SUP4t (Plasmid #43803) from Dr. Church lab.
• Vector type: CRISPR ; gRNA
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gRNA for clr-2 locus
• Alt name: gRNA
• Alt name: clr-2
• gRNA/shRNA sequence: GAGTGCCCTAGTCGGTGTGA
• Species: N. crassa
• Tag / Fusion Protein:
  • No

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GGAGCTTCTTTGAAAAGATAATGTATG
• 3′ sequencing primer: GTTACATGCGTACACGCGTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry