pFosill-3
(Plasmid
#89697)
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Purpose(Empty Backbone) to make Fosmids that can be converted into Illumina sequencable libraries
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFOS1
- Backbone size (bp) 9738
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Modifications to backboneRemoved SapI sites from backbone. Inserted Nb.BbvCI nicking sites, SBS3 and SBS8 Illumina sequences into cloning site.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFosill-3 was a gift from Andreas Gnirke (Addgene plasmid # 89697 ; http://n2t.net/addgene:89697 ; RRID:Addgene_89697) -
For your References section:
Paired-end sequencing of Fosmid libraries by Illumina. Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke A. Genome Res. 2012 Nov;22(11):2241-9. doi: 10.1101/gr.138925.112. Epub 2012 Jul 16. 10.1101/gr.138925.112 PubMed 22800726
