pAd/CMV/V5-Clasp2α
(Plasmid #89763)

• Purpose: Adenovirus destination vector for transient expression of human wild-type EGFP-CLASP2α
• Depositing Lab: Torsten Wittmann
• Publication: Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412. doi: 10.1242/jcs.194662. Epub 2017 Feb 23.

Backbone

• Vector backbone: pAd/CMV/V5-DEST
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 35500
• Total vector size (bp): 40000
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CLASP2α
• Alt name: CLASP2
• Species: H. sapiens (human)
• Insert Size (bp): 4500
• GenBank ID: NM_015097.2
• Entrez Gene: CLASP2
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP_C
• 3′ sequencing primer: V5_reverse

Resource Information

• Supplemental Documents:
  • pHP45_pAd_CMV_V5DEST_EGFP_CLASP2_alpha.gbk
• A portion of this plasmid was derived from a plasmid made by: CLASP2α sequence was from Irina Kaverina

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CLASP2α adenovirus vectors were also used in these publications:

https://www.ncbi.nlm.nih.gov/pubmed/24923837
https://www.ncbi.nlm.nih.gov/pubmed/25231989