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pRK7-HA-S6K1-E389
(Plasmid #8987)

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Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    S6K1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1500
  • Mutation
    T389E
  • Entrez Gene
    Rps6kb1
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

E389 mimics Thr-389 phosphorylation.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK7-HA-S6K1-E389 was a gift from John Blenis (Addgene plasmid # 8987)
  • For your References section:

    Identification of a conserved motif required for mTOR signaling. Schalm SS, Blenis J. Curr Biol. 2002 Apr 16. 12(8):632-9. 10.1016/S0960-9822(02)00762-5 PubMed 11967149