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Plasmid 8987: pRK7-HA-S6K1-E389
  • S6K1

  • 1500

  • R. norvegicus (rat)

  • Rps6kb1 ()

  • HA

  • N terminal on insert

  • T389E

  • pRK7
    (Search Vector Database)

  • Mammalian Expression

  • 4700

  • XbaI

  • No

  • EcoRI

  • No

  • SP6 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (3)
  • John Blenis

  • UBMTA

Comments: 

E389 mimics Thr-389 phosphorylation.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Identification of a conserved motif required for mTOR signaling. Schalm et al (Curr Biol. 2002 Apr 16. 12(8):632-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8987" in your Materials and Methods section.

Discontinued

Addgene no longer distributes this plasmid. Contact help@addgene.org for more information