Purpose(Empty Backbone) Linker to construct BB2 from BB1(FS1 - FS4) with FSE and FSF (in with BbsI, out with BsaI)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89921||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2215
Vector typeBacterial Expression
Growth in Bacteria
- Cloning method Unknown
- 5′ sequencing primer CATTAATGCAGCTGGCAC
- 3′ sequencing primer GGTTATTGTCTCATGAGCGG (Common Sequencing Primers)
Addgene's sequencing results found that this vector is larger than other BB2 vectors. The depositing lab confirmed that, since the vector contains FS E and FS F sites, it should be functional for cloning purposes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BB2_L_EF_syn_BbsI was a gift from Michael Sauer (Addgene plasmid # 89921 ; http://n2t.net/addgene:89921 ; RRID:Addgene_89921)
For your References section:An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger. Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG. Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. 10.1016/j.biortech.2017.05.004 PubMed 28533066