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pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
(Plasmid #90009)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90009 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSF3
  • Total vector size (bp) 8585
  • Vector type
    Mammalian Expression, Retroviral ; Flp/FRT
  • Selectable markers
    Hygromycin ; Ganciclovir (negative selection when making stable cell lines)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    FKBP-linker-Myc-NBirA*
  • Insert Size (bp)
    1233
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    FRB-linker-CBirA*
  • Insert Size (bp)
    627
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmids: pLXIN-Ap1g1-FKBP (#46946) and pEYFP-Mitotrap (#46942) kind gifts from Margaret Robinson and pCDNA3.1-MycBioID (#35700) kind gift from Kyle Roux

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir was a gift from Julien Béthune (Addgene plasmid # 90009 ; http://n2t.net/addgene:90009 ; RRID:Addgene_90009)
  • For your References section:

    Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild K, Bethune J. Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690. 10.1038/ncomms15690 PubMed 28585547