|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90100||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 11457
Modifications to backbonepPtPBR1 (episomal maintenance in the diatom P. tricornutum) was modified to include 49202 promoter, (AttR1-CatR-ccdB-AttR2), and FcpA terminator.
Vector typeGateway Destination vector for protein expression in diatoms
- Promoter 49202
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, Ampicillin, Tetracycline
Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p49202-DEST was a gift from Philip Weyman (Addgene plasmid # 90100 ; http://n2t.net/addgene:90100 ; RRID:Addgene_90100)