Addgene - 1130 pBabe puroL HA FKHR H215R AAA Plasmid Data

Plasmid 9035: 1130 pBabe puroL HA FKHR H215R AAA

Gene/insert name:
FKHR H215R AAA
Alt name:
FKHR
Alt name:
FOXO1A
Insert size:
2000
Species:
H. sapiens (human)
Entrez Gene:
FOXO1 (FKH1, FKHR, FOXO1A)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Mutation:
H215R T24A S256A S319A
Vector backbone:
pBabe puro L
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5200
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
pBabe5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Comments:

Oligonucleotides 5'-GCGCGGATCCATGGCCGAGGCGCCTCAGGTG-3' and 5'-CGCGCTCGAGGAATTCTCAGCCTGACACCCAGCTATG-3' were used to PCR amplify the FKHR cDNA. The PCR products, restricted with BamHI and XhoI, were ligated to similarly restricted pBABE-puroL to give pBABE-puroL-FKHR.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR. Ramaswamy et al (Cancer Cell. 2002 Jul . 2(1):81-91. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9035" in your Materials and Methods section.