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pBABE-neo largeTcDNA H42Q
(Plasmid #9048)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBABE-neo
  • Backbone size w/o insert (bp) 5330
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    large T antigen
  • Alt name
    large T cDNA
  • Alt name
    SV40gp6
  • Alt name
    LTc
  • Species
    simian virus
  • Insert Size (bp)
    2200
  • Mutation
    H42Q. Renders J domain nonfunctional.
  • Entrez Gene
    SV40gp6 (a.k.a. SV40gp6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-neo largeTcDNA H42Q was a gift from Bob Weinberg (Addgene plasmid # 9048)
  • For your References section:

    Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, DeCaprio JA, Weinberg RA. Mol Cell Biol 2002 Apr;22(7):2111-23. 10.1128/MCB.22.7.2111-2123.2002 PubMed 11884599