Addgene - pBABE puro H-Ras V12 Plasmid Data

Plasmid 9051: pBABE puro H-Ras V12

Gene/insert name:
Hras V12
Alt name:
v-Ha-ras
Alt name:
Hras
Insert size:
700
Species:
H. sapiens (human)
Entrez Gene:
HRAS (C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1)
Mutation:
G12V, constitutively active
Vector backbone:
pBabe puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE-5 List of Sequencing Primers
3' sequencing primer:
pBABE-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
Scott Lowe, Robert Weinberg
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
William Hahn
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9051" in your Materials and Methods section.