pBABE-hygro p53 DD
(Plasmid #9058)

• Depositing Lab: Bob Weinberg
• Publication: Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Backbone

• Vector backbone: pBABE hygro
• Backbone size w/o insert (bp): 5558
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53 DD
• Alt name: p53
• Species: M. musculus (mouse)
• Insert Size (bp): 309
• Mutation: DD. Dominant negative mutant carries deletion of 288 amino acids (D15-301), leaving initial 14aa and the oligomerization and COOH-domains.
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: blunt (destroyed during cloning)
• 5′ sequencing primer: pBABE-5
• 3′ sequencing primer: pBABE-3

Resource Information

• Addgene Notes:
  • Updated sequence information
• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry