Addgene - pMIG small T H42Q Plasmid Data

Plasmid 9062: pMIG small T H42Q

Gene/insert name:
SV40 small T Antigen H42Q
Alt name:
SV40 small T Antigen
Insert size:
520
Species:
simian virus
Entrez Gene:
SV40gp7 (SV40gp7)
Fusion protein or tag:
IRES GFP
Terminal:
C terminal on backbone
Mutation:
H42Q. Renders J domain nonfunctional.
Vector backbone:
pMIG
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
7000
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

A retrovirus encoding the SV40 small t (ST) oncoprotein was constructed by amplifying the ST sequences by PCR using the oligonucleotides B5ST (5' GGCGGATCCGCCACCATGGATAAAGTTTT 3') and E3ST (5'AGGCGAATTCTTAGAGCTTTAAATCTCTG 3'). The resulting fragment was sequenced and introduced into pMIG. H42Q mutant.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Hahn et al (Mol Cell Biol 2002 Apr;22(7):2111-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9062" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

9059: pMIG small T

9060: pMIG small T C97S E102Q

29559: hCent2-pEGFP-C1