Addgene - pBABE-zeo small T H42Q Plasmid Data

Plasmid 9065: pBABE-zeo small T H42Q

Gene/insert name:
SV40 small T Antigen
Insert size:
520
Species:
simian virus
Entrez Gene:
SV40gp7 (SV40gp7)
Vector backbone:
pBABE-zeo
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
4800
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

A retrovirus encoding the SV40 small t (ST) oncoprotein was constructed by amplifying the ST sequences by PCR using the vector pVU- (30) and the oligonucleotides B5ST (5' GGCGGATCCGCCACCATGGATAAAGTTTT 3') and E3ST (5'AGGCGAATTCTTAGAGCTTTAAATCTCTG 3'). The resulting fragment was sequenced and introduced into pMIG (pMIG-ST) (81) and pBABE-zeo. H42Q mutant.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Hahn et al (Mol Cell Biol 2002 Apr;22(7):2111-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9065" in your Materials and Methods section.