NEK2 A5.4 gRNA
Purpose3rd generation lentiviral gRNA plasmid targeting human NEK2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90796||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerEric Lander & David Sabatini labs (Addgene plasmid #71409)
- Backbone size w/o insert (bp) 9151
Vector typeLentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert nameNEK2 (Guide Designation A5.4)
Alt namepKMKO series
SpeciesH. sapiens (human)
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer hU6-F (5'-GAGGGCCTATTTCCCATGATT-3')
- 3′ sequencing primer EF1a-R (5'-CACGGCGACTACTGCACTTA-3') (Common Sequencing Primers)
Please note that gRNA target sequence listed is not strictly the 20nt spacer sequence, but also contains the 5' BsmBI overhang. For more information about knockout human cell lines generated using this plasmid, please see http://cellcycleknockouts.wi.mit.edu/ .
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NEK2 A5.4 gRNA was a gift from Iain Cheeseman (Addgene plasmid # 90796 ; http://n2t.net/addgene:90796 ; RRID:Addgene_90796)
For your References section:Large-Scale Analysis of CRISPR/Cas9 Cell-Cycle Knockouts Reveals the Diversity of p53-Dependent Responses to Cell-Cycle Defects. McKinley KL, Cheeseman IM. Dev Cell. 2017 Feb 27;40(4):405-420.e2. doi: 10.1016/j.devcel.2017.01.012. Epub 2017 Feb 16. 10.1016/j.devcel.2017.01.012 PubMed 28216383